1. Number of flagella
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0, none
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1, one
|
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2, multiple
|
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2. Electron-opaque plug in axoneme core and between axoneme and flagellar membrane
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0, absent
|
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1, present
|
|
3. Flagellum coating
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0, absent
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1, present
|
|
4. Electron-opaque core in kinetosome
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0, absent
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1, present
|
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5. Scalloped ring within kinetosome, extensions of the A, B, or C microtubule
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0, absent
|
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1, present (Lacustromyces hiemalis)
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|
6. Kinetosome support
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0, absent
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1, kinetosome props
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2, broken kinetosome props
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3, skirt-like structure surrounding kinetosome (Neocallimastigomycota)
|
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7. Kinetosome-associated plates
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0, absent
|
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1, present (Group I type zoospore [Barr 1980])
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8. Kinetosome-associated saddle
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0, absent
|
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1, present (Group II type zoospore [Barr 1980])
|
|
9. Kinetosome-associated shield
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0, absent
|
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1, present
|
|
10. Kinetosome-associated spur
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0, absent
|
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1, present
|
|
11. Kinetosome-associated striated disc
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0, absent
|
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1, present (Chytridiomycota: Monoblepharidomycetes)
|
|
12. Primary microtubule root
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0, absent
|
|
1, 2-5 laterally-oriented, parallel, stacked microtubules arising from side of kinetosome (Chytridiomycota: Chytridiomycetes: Rhizophydiales- Alphamyces, Aquamyces, Globomyces, Urceomyces, Gorgonomyces, Pateramyces, Protrudomyces, Rhizophydium, Terramyces, Coralloidiomyces)
|
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2, approx. 6-7 laterally-oriented, parallel, bundled microtubules arising from side of kinetosome (Chytridiomycota: Chytridiomycetes: Chytridiales)
|
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3, more than 20 laterally-oriented, parallel, bundled microtubules with linkers between them, arising from side of kinetosome (Chytridiomycota: Chytridiomycetes: Cladochytriales)
|
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4, multiple microtubules extending anteriorly from kinetosome, parallel to kinetosome triplets (Chytridiomycota: Chytridiomycetes: Rhizophydiales- Batrachochytrium)
|
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5, microtubules in cone arising from a spur at the anterior end of kinetosome (Chytridiomycota: Chytridiomycetes: Spizellomycetales- Spizellomyces)
|
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6, microtubules extending anteriorly without regular arrangement from a spur on the kinetosome (Chytridiomycota: Chytridiomycetes: Spizellomycetales- Kochiomyces)
|
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7, microtubules in cone arising from a double bar at the anterior end of kinetosome (Chytridiomycota: Chytridiomycetes: Spizellomycetales- Gaertneriomyces)
|
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8, microtubules in cone arising from two concave discs at the anterior end of kinetosome (Chytridiomycota: Chytridiomycetes: Spizellomycetales- Powellomyces)
|
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9, microtubules extending parallel and anteriorly from kinetosome and adjacent to an elongated tripartite spur (Chytridiomycota: Chytridiomycetes: Spizellomycetales- Triparticalcar)
|
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a, root of two parts: a striated disk extending part way around kinetosome, and microtubules extending anteriorly from kinetosome (Chytridiomycota: Monoblepharidomycetes)
|
|
b, up to 27 microtubules in groups of 3 extending anteriorly from kinetosome (Blastocladiomycota)
|
|
c, multiple microtubules emanating from spurs on multiple kinetosomes (Neocallimastigomycota)
|
|
13. Primary microtubule root absolute configuration
|
0, absent
|
|
1, 20 degrees left average (10-35 degrees)
|
|
2, 10 degrees right average (0-25 degrees)
|
|
3, 50 degrees left average (20-80 degrees)
|
|
14. Striated rhizoplast between kinetosome and nucleus
|
0, absent
|
|
1, present (Rhizophlyctis rosea, Sonoraphlyctis ranzonii, Caulochytrium protostelioides, Olpidium brassicae)
|
|
15. Non-flagellated centriole
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0, absent (incertae sedis: Thalassochytrium gracilariopsidis)
|
|
1, parallel to kinetosome
|
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2, oblique to kinetosome
|
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3, at right angle to kinetosome
|
|
16. Length of non-flagellated centriole (NfC) compared to length of kinetosome (K)
|
0, NfC < K (Chytridiomycota: Monoblepharidomycetes; Chytridiomycetes: Rhizophydiales- Kappamyces; Chytridiales)
|
|
1, NfC = K
|
|
2, NfC > K (Chytridiomycota: Chytridiomycetes: Polychytrium clade- Lacustromyces hiemalis)
|
|
17. Kinetosome-non-flagellated centriole fibrillar bridge
|
0, absent
|
|
1, fibers perpendicular to NfC and K
|
|
2, fibers parallel to NfC and K
|
|
18. Non-flagellated centriole-associated veil
|
0, absent
|
|
1, present (Chytridiomycota: Chytridiomycetes- Chytridiales)
|
|
19. Paracrystalline inclusion
|
0, absent
|
|
1, present (Chytridiomycota: Chytridiomycetes- Chytridiales, Polychytrium clade; ?Blastocladiomycota: Catenaria anguillulae)
|
|
20. Ribosomal organization
|
0, scattered ribosomes (Chytridiomycota: Chytridiomycetes: Spizellomycetales; Rhizophlyctidales- Rhizophlyctis rosea, Sonoraphlyctis ranzonii; incertae sedis: Synchytrium macrosporum, Caulochytrium protostelioides)
|
|
1, core area of aggregated ribosomes partially or completely enclosed by endoplasmic reticulum (Chytridiomycota: Chytridiomycetes: Chytridiales, Rhizophydiales, Cladochytriales, Lobulomycetales; incertae sedis: Polyphagus euglenae; Monoblepharidomycetes)
|
|
2, aggregated as a nuclear cap (Blastocladiomycota)
|
|
3, loosely enclosed by endoplasmic reticulum and surrounding nucleus (Chytridiomycota: Chytridiomycetes: Rhizophlyctidales- Arizonaphlyctis, Borealophlyctis)
|
|
21. Mitochondrion
|
0, absent (hydrogenosomes: Neocallimastigomycota)
|
|
1, single
|
|
2, multiple
|
|
22. Cisterna
|
0, absent
|
|
1, simple
|
|
2, fenestrated
|
|
3, annular
|
|
23. Paraxonemal structure
|
0, absent
|
|
1, present (Chytridiomycota: Monoblepharidomycetes- Gonapodya polymorpha)
|
|
24. Water expulsion vacoule
|
0, absent (Chytridiomycota: Chytridiomycetes- Chytridiales)
|
|
1, present (Chytridiomycota: Chytridiomycetes- Spizellomycetales; Monoblepharidomycetes)
|
|
25. Golgi apparatus morphology
|
0, stacked cisternae (Chytridiomycota; Blastocladiomycota- Physoderma)
|
|
1, absence of stacked cisternae (Blastocladiomycota)
|
|
26. Golgi apparatus location
|
0, absent
|
|
1, associated with flagellar rootlet(Chytridiomycota: Chytridiomycetes- Chytridiales)
|
|
2, not associated with flagellar rootlet
|
|
27. Relationship of microbodies to lipid(s) or nucleus
|
0, microbody associated with a single lipid (Chytridiomycota: Chytridiomycetes- Chytridiales, Cladochytriales, Lobulomycetales, Rhizophydiales)
|
|
1, microbody associated with multiple lipids (Chytridiomycota: Chytridiomycetes: Spizellomycetales, Rhizophlyctidales, Rhizophydiales- Batrachochytrium; incertae sedis: Caulochytrium protostelioides)
|
|
2, microbody associated with nucleus (Chytridiomycota: Chytridiomycetes: Rhizophlyctidales- Rhizophlyctis rosea, Sonoraphlyctis ranzonii)
|
|
28. Location of cisterna relative to lipid(s)
|
0, adjacent to lipids (Chytridiomycota: Chytridiomycetes: Chytridiales, Rhizophydiales, Cladochytriales)
|
|
1, backs microbody (Blastocladiomycota)
|
|
2, fenestrated cisterna (rumposome) backed by microbody (Chytridiomycota: Monoblepharidomycetes)
|
|
3, absent (Chytridiomycota: Chytridiomycetes: Rhizophydiales- Batrachochytrium; Polychytrium clade- Lacustromyces hiemalis)
|
|
29. Location of mitochondria
|
0, outside ribosomal core
|
|
1, within ribosomal core
|
|
2, scattered in cytoplasm
|
|
30. Nuclear heel
|
0, absent
|
|
1, present (Chytridiomycota: Chytridiomycetes: Spizellomycetales- Spizellomyces; Rhizophlyctidales- Sonoraphlyctis, Borealophlyctis)
|
|
31. Nucleus location
|
0, distal to kinetosome
|
|
1, proximal to kinetosome
|
|
2, connected to kinetosome
|
|
32. Posterior dome composed of peripheral granules and array of megatubules
|
0, absent
|
|
1, present (Neocallimastigomycota)
|
|