|
1. Spitzenkörper (SPK)
|
0, absent
|
|
1, present
|
|
|
2. Vesicles concentrated in thin layer beneath apical plasma membrane and/or loosely organized throughout apical dome
|
0, absent
|
|
1, present
|
|
|
3. SPK function
|
1, SPK not nucleating microtubules
|
|
2, SPK nucleating microtubules
|
|
0, SPK absent
|
|
|
4. SPK structure (light microscopy)
|
1, central core absent or not visible
|
|
2, central core present, phase-bright
|
|
3, central core present, phase-dark
|
|
0, SPK absent
|
|
|
5. SPK detailed structure (electron microscopy)
|
1, central core absent
|
|
2, central core enriched with microvesicles
|
|
3, central core with few or no microvesicles
|
|
0, SPK absent
|
|
|
6. Ribosome cluster distal to SPK or vesicle accumulation in apical dome
|
0, absent or not observed
|
|
1, present
|
|
|
7. Macrovesicles with uniformly dense lumen
|
0, absent
|
|
1, present, 70–120 nm diam.
|
|
2, present, 140–250 nm diam.
|
|
|
8. Microvesicles (25–45 nm diam.) with uniformly dense lumen
|
0, absent
|
|
1, present (smooth profile)
|
|
2, present (polyhedral profile)
|
|
|
9. Macrovesicles (90–250 nm diam.) with an electron dense core, and less dense periphery
|
0, absent
|
|
1, present
|
|
|
10. Microvesicles with an electron dense core, and less dense periphery (30-65 nm diam.)
|
0, absent
|
|
1, present
|
|
|
11. Filasomes (protein-coated microvesicles; 30–40 nm diam.)
|
0, absent
|
|
1, present but uncommon
|
|
2, present and common
|
|
|
12. Sub-apical cluster of putative lipid globules
|
0, absent
|
|
1, present
|
|
